This cardiac tissue was part of a large project I worked on for UCSF and Stanford. It was a lot of work and I'm really excited to present results of our microscopy methods project at ASCB. Come check it out on Sunday in the Light and Electron Microscopy poster session.
All my scope time right now feels like a sneaky luxury
I caught up with a friend who I hadn't seen in a while and started chatting with her awesome kids. Her daughter was wearing an awesome dress with a popsicle pattern; before I could stop myself I complimented her on it. I try to never start conversations with girls by commenting on their appearances so I quickly moved to asking about what good books they'd both read recently.
We got to chatting some more and I was talking about my lab work and how I use a lot of test tubes. She jumped on the number I mentioned and in that fantastic, hyperbolic way kids speak and said, "I've never seen that many test tubes in my whole life!"
It got me thinking about how many test tubes I do use. I counted 52 for my most recent experiment and mentioned this to her mom on facebook. Her mom replied that her daughter referred to me as Test Tube Lady. Which, yes, yes I am.
More diatoms! Sadly, the desktop SEM went back home!
Weird, the DAPI channel didn't come out.
I was imaging some ocean kelp with the visiting desktop SEM and look what showed up! It was covered in diatoms! I have so many new kids to look up now. I haven't seen the snow-shoe looking ones before. Very exciting find!
So this is annoying. The last few tiling runs I've attempted have all come out looking like this. <img src="http://oikosjournal.files.wordpress.com/2011/05/solved-smiley-face1.jpg">
I tried tiling post capture but no luck! Wonder what I'm missing... Guess I could cut it up in PS and put it together again. I do like puzzles!
I wonder how people did this before image software did it for you or allowed you to move the pieces around easily?
These are so challenging and fun.
I found a slide in a box simply labeled "Mixed Bacteria" and of course I had to put it in my confocal and see what was there. These are some cute and standard shaped rods, so I'm guessing Bacilus.
I'm always sharking scope systems in the hope that I can learn how to use them. It's often trial and error if the set up or software isn't immediately easy to understand. I've definitely spent more time than I'd like hunting for standard features that seem hidden under clickable triangles or headers with barely legible text (why such small fonts?). I forgot to label this image because I spent a frustrating hour trying to get SOMETHING to appear. I'm usually very good with my image log, but ugh what I managed to get didn't seem worth capturing. I'm pretty sure this my ephyra slide. It's a max projection giving that three dimensional appearance.
Of course, normally this is a facet of microscopy that I love. It really does feel like magic to tweak the settings, adjust the laser (mmm lasers), open the pinhole and then suddenly the pixels light up and there it is!
I'm looking forward to trying out this system again with fresh eyes.